烟曲霉
白色念珠菌
生物
微生物学
白色体
免疫系统
分泌物
先天免疫系统
免疫学
生物化学
作者
Daniela Langenhorst,Anna-Lisa Fürst,Karl Alberter,Cláudia Vilhena,Prasad Dasari,Muhammad Daud,Linda Heilig,Christian H. Luther,Marcus Dittrich,Nadine Reiher,Melissa Wich,Mohammed El‐Mowafy,Ilse D. Jacobsen,Berit Jungnickel,Peter F. Zipfel,Niklas Beyersdorf
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:2023-07-12
卷期号:211 (5): 804-815
被引量:1
标识
DOI:10.4049/jimmunol.2200318
摘要
Because of the growing numbers of immunocompromised patients, the incidence of life-threatening fungal infections caused by Candida albicans and Aspergillus fumigatus is increasing. We have recently identified enolase 1 (Eno1) from A. fumigatus as an immune evasion protein. Eno1 is a fungal moonlighting protein that mediates adhesion and invasion of human cells and also immune evasion through complement inactivation. We now show that soluble Eno1 has immunostimulatory activity. We observed that Eno1 from both C. albicans and A. fumigatus directly binds to the surface of lymphocytes, preferentially human and mouse B cells. Functionally, Eno1 upregulated CD86 expression on B cells and induced proliferation. Although the receptor for fungal Eno1 on B lymphocytes is still unknown, the comparison of B cells from wild-type and MyD88-deficient mice showed that B cell activation by Eno1 required MyD88 signaling. With respect to infection biology, we noted that mouse B cells stimulated by Eno1 secreted IgM and IgG2b. These Igs bound C. albicans hyphae in vitro, suggesting that Eno1-induced Ab secretion might contribute to protection from invasive fungal disease in vivo. Eno1 also triggered the release of proinflammatory cytokines from monocytes, particularly IL-6, which is a potent activator of B cells. Together, our data shed new light on the role of secreted Eno1 in infections with C. albicans and A. fumigatus. Eno1 secretion by these pathogenic microbes appears to be a double-edged sword by supporting fungal pathogenicity while triggering (antifungal) immunity.
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