Staphylococcus aureusColony Polymerase Chain Reaction

Here, we describe a protocol for a colony polymerase chain reaction (PCR) method for Staphylococcus aureus . The methodology involves the preparation of small S. aureus lysates by using the enzyme lysostaphin to degrade the peptidoglycan layer. These lysates are prepared using a small patch of bacteria grown on LB agar plates, and the lysates can subsequently be used for PCR analyses.