Integration of Hyphenated Techniques for Characterizing and Chemical Profiling of Natural Products

ABSTRACT The drug discovery from natural products has played an important role for therapeutic purposes, however, but in the past two decades, there has been a hurdle faced by researchers during purification and characterization. Traditional analytical approaches are insufficient to address the growing number of difficulties in natural product research. The hyphenated approach is a more advanced form that combines the benefits of separation with spectroscopy. The exceptional advancements in hyphenated systems have significantly expanded their applications in the investigation of natural products. The advancement of numerous chromatographic techniques such as HPLC, HPTLC, and GC, as well as spectroscopic techniques such as NMR, MS, FTIR, and UV, in addition to the advent of hyphenated techniques such as LC‐MS, GC‐MS, and LC‐NMR, have significantly transformed the method of drug discovery from natural resources. This study discusses the general concepts and literature applications of productivity tools for natural product isolation and structural elucidation. These hyphenated methodologies will enhance the course of natural product research while reducing the time and cost invested in its investigation, hence speeding up the drug development process.