The effects of gamma-aminobutyric acid on growth performance, diarrhea, ruminal fermentation, and antioxidant capacity in pre-weaned calves

The pre-weaning phase is a vital period for the growth and development of calves, significantly impacting their future health and productivity. Gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter in the mammalian central nervous system that lowers blood pressure, stimulates feed intake, and enhances antioxidant capability. Gamma-aminobutyric acid has been proven beneficial for adult cows, while little research has been conducted on calves. Therefore, this study examined the effects of GABA on growth performance, diarrhoea, ruminal fermentation, and antioxidant capacity in pre-weaned Holstein calves. Ninety male Holstein calves were allocated to five groups: 0 mg/d (G0), 25 mg/d (G25), 50 mg/d (G50), 100 mg/d (G100), and 200 mg/d (G200). The experiment was conducted from 11 to 75 days of calves age, and the calves were weaned at 75 days of age. Growth performance indicators, ruminal fluid, faecal score, and serum were collected at 11, 28, 42, 60, and 75 days of calves' age. The results showed that adding GABA positively affected average daily gain and body height, with no effects on diarrhoea frequency. All dosages significantly reduced acetate and total volatile fatty acid levels in ruminal fermentation, with butyrate showing a complex response at higher doses. Overall, we recommended 100 mg/d as the optimal GABA supplementation level to improve growth performance and regulate the ruminal fermentation of pre-weaned calves before weaning.