CpG寡核苷酸
免疫系统
免疫学
支气管肺泡灌洗
哮喘
CpG站点
巨噬细胞
医学
气道
巨噬细胞极化
生物
肺
基因表达
体外
基因
内科学
外科
DNA甲基化
生物化学
作者
Huanping Zhang,Lihuan Wang,Aizhi Zhang,Xiangyu Wang,Yun Liao,Xiaoxue Chen,Xuejie Xu,Lei Yang,Yu Liu,Aifa Tang,Ping‐Chang Yang
摘要
Allergic asthma is characterized by the polarization of Th2 cells and impaired immune regulation. Macrophages occupy the largest proportion of airway immune cells. This study aims to discover the mechanism that hinders the immune regulatory functions of airway macrophages. In this study, macrophages were isolated from cells in bronchoalveolar lavage fluids (BALF) collected from asthma patients and normal control (NC) subjects. The results indicated that macrophages occupied the largest portion of the cellular components in BALF. The frequency of IL-10+ macrophage was significantly lower in asthma patients than in NC subjects. The expression of IL-10 in macrophages of BALF was associated with the levels of asthma-related parameters. The immune-suppressive functions of BALF M0 cells were defective in asthma patients. The inducibility of IL-10 expression was impaired in BALF macrophages of asthma patients, which could be restored by exposing to CpG. In conclusion, the induction of IL-10 in macrophages of BALF in asthma patients was impaired, and it could be restored by exposure to CpG.
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