生物相容性
脂滴
荧光
化学
细胞器
生物物理学
咔唑
细胞毒性
脂质代谢
光化学
咪唑
纳米技术
材料科学
粘度
生物化学
有机化学
体外
光学
生物
复合材料
物理
作者
Huiying Chen,Jianzhi Zhao,Junzhi Lin,Baoli Dong,Hui Li,Bo Geng,Mei Yan
出处
期刊:RSC Advances
[The Royal Society of Chemistry]
日期:2021-01-01
卷期号:11 (14): 8250-8254
被引量:5
摘要
Lipid droplets (LDs) are storage organelles at the centre of lipid and energy homeostasis, which act as vital hubs of cellular metabolism and the key to maintaining lipid and energy homeostasis. We synthesized a new two-photon fluorescent probe (CIV) that could detect the viscosity of lipid droplets. The probe is constructed via the typical ICT system of D-π-A using carbazole as the donor and imidazole as the acceptor. With the increase in viscosity from PBS to 99% glycerol, the fluorescence intensity of CIV increased by 13-fold, showing sensitivity and specificity towards viscosity. In addition, CIV showed low toxicity and excellent biocompatibility in cytotoxicity tests, and was successfully used for living cell LD imaging. Taken together, the results widen the way for the development of novel fluorescent probe-based the visualization LDs and detection in solutions, physiology and pathology.
科研通智能强力驱动
Strongly Powered by AbleSci AI