重组酶聚合酶扩增
环介导等温扩增
病毒学
聚合酶链反应
实时聚合酶链反应
生物
分子生物学
计算生物学
计算机科学
遗传学
DNA
基因
作者
Xiaohan Yang,Jingyu Xie,Siqi Hu,Wenli Zhan,Lei Duan,Keyi Chen,Changbin Zhang,Aihua Yin,Mingyong Luo
标识
DOI:10.1016/j.snb.2020.127903
摘要
Enteroviruses (EVs) are the most common causative pathogen of infection in children aged under 5 years. Routine laboratory methods for detecting EV are time consuming, labor intensive, and require sophisticated thermal cycling instruments and skilled operators, which are not available in limited-resource settings. In this study, a novel isothermal amplification, recombinase polymerase amplification (RPA) combined with lateral flow strips (LFS), was established to detect EVs. Specific RPA-LFS primers and probe were designed to target the highly conserved regions of 5′-UTR. The analytical sensitivity for detection of EV was 5 copies per reaction, with 100 % specificity. The clinical performance was evaluated using 177 clinical samples, and the coincidence rates between RPA and commercial quantitative real-time PCR was 100 %. In conclusion, the RPA-LFS developed in this study is a rapid, specific, sensitive, and accurate assay for detecting EV and could thus be an ideal diagnostic tool for EV infections in limited-resource settings. It is the first time that RPA-LFS assay has been applied to the detection of EV infection.
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