Red Blood Cell Lysis Protocols v1

BioLegend’s Red Blood Cell (RBC) Lysis Buffer (Cat. No. 420301) has been designed,formulated, and tested to ensure optimal lysis of RBCs in single cell suspensionsof hematopoietic tissues, such as spleen, as well as RBCs derived from peripheralblood. RBC Lysis Buffer contains ammonium chloride, which has been demonstratedto lyse RBCs with a minimum of harmful effects on lymphocytes