Effect of sperm concentration on boar spermatozoa mitochondrial membrane potential and motility in semen stored at 17 °C

The aim of the study was to assess the effect of sperm concentration in the ejaculate on the mitochondrial membrane potential and motility of Landrace boar spermatozoa during storage of diluted semen at 17 °C. The study was conducted on ejaculates collected from 10 boars aged 1.5–2 years. Based on sperm concentration measurements, two groups of boars were identified: Group 1 – boars providing ejaculates with a sperm concentration of at least 500 × 10 3 /mm 3 and Group 2 – boars providing ejaculates with a sperm concentration of less than 500 × 10 3 /mm 3 . Four ejaculates were collected manually from each boar. Each ejaculate was diluted with Biosolvens Plus diluent, and insemination doses were prepared and stored at 17 °C. Mitochondrial membrane potential and motility of spermatozoa were evaluated at each insemination dose. The tests were carried out after 1, 24, 48, 96 and 168 h of storage. Based on the results, it was found that ejaculates with a sperm concentration ≥ 500 × 10 3 /mm 3 have a lower share of spermatozoa with high mitochondrial membrane potential than ejaculates with a sperm concentration below 500 × 10 3 /mm 3 . A high correlation between the share of spermatozoa with a high mitochondrial membrane potential and motility of spermatozoa was demonstrated in the first 24 h and after 96 h of semen storage, which was confirmed by the calculated phenotypic correlation coefficients. Sperm cells in ejaculates with a higher sperm concentration are more sensitive to storage time than spermatozoa in ejaculates with a lower concentration.