化学
荧光
细胞内pH值
平衡
生物物理学
线性范围
分析化学(期刊)
细胞内
色谱法
检出限
生物化学
量子力学
生物
细胞生物学
物理
作者
Qian Liu,Miao Yang,Xiangchuan Meng,Xiao Han,Marc Nazaré,Youjun Xu,Hai‐Yu Hu,Qingyang Zhang
出处
期刊:Talanta
[Elsevier]
日期:2022-08-01
卷期号:246: 123493-123493
被引量:4
标识
DOI:10.1016/j.talanta.2022.123493
摘要
pH homeostasis is essential for alkaliphiles, given their widespread use in biotechnological applications. However, quantitative monitoring of alkaline pH in alkaliphiles remains challenging. Here, we synthesized for the first time, a thermally activated delayed fluorescent (TADF) pH probe: NI-D-OH. Our probe exhibits a good linear relationship between fluorescence intensity and pH in the neutral to alkaline range (pH 7.0-8.6), as well as long-lived TADF emission. We further show that NI-D-OH can be used to monitor intracellular pH in living organisms, and evaluate the effect of Na+ on the pH homeostasis, demonstrating the potential for alkaline pH monitoring and time-resolved fluorescence imaging.
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