Study on susceptibility of Acinetobacter baumannii to common disinfectants

Objective To study the susceptibility of Acinetobacter baumannii to common disinfectants and to provide more practical measures for hospital disinfection. Methods The susceptibilities of multidrug- resistant Acinetobacter baumannii to three common disinfectants in hospital were evaluated by determination of minimum bactericidal concentration( MBC) and minimum inhibitory concentration( MIC). Disinfectant resistance gene qac EΔ1- sull was detected by PCR.Results The MBCs of formaldehyde disinfectant,glutaraldehyde disinfectant and 2% strengthening glutaraldehyde disinfectant against multidrug- resistant Acinetobacter baumannii were 500,20 000 and 2 500 mg / L,respectively,while the MICs were 250,5 000 and 315 mg / L,respectively. The MBCs of formaldehyde disinfectant,glutaraldehyde disinfectant and 2% strengthening glutaraldehyde disinfectant against sensitive Acinetobacter baumannii and standard strains were 250,10 000 and 1 250 mg / L,respectively,while the MICs were 125,2 500 and 78 mg / L,respectively. The qac EΔ1- sull gene was detected in 7 multiple drug resistant Acinetobacter baumannii strains,while not in 5 strains of sensitive strains of Acinetobacter baumannii and quality controls. Conclusion The MBC and the MIC of multidrug- resistant Acinetobacter baumannii to disinfectants are significantly increased. Multiple drug resistant Acinetobacter baumannii resistance to disinfectant is associated with disinfectant resistance genes qac EΔ1- sull.