[Objective]The aim was to provide a reference for the application of straw bio-reactor technology.[Method] The effects of straw species on application of straw bio-reactor technology were elaborated,by carrying out a different types of application effect of straw test for corn stalks and rice straw.[Result] Applying bio-reactor on cucumber with facility cultivation,the yield of applying corn stalks increased by 5.26% than applying rice straw,and the output value increased by 5.55%.Applying bio-reactor on tomato with facility cultivation,the yield of applying corn stalks increased by 5.26% than applying rice straw,and the output value increased by 5.55%.[Conclusion] Applying bio-reactor technology on cucumber and tomato production in greenhouse,the yield increased by applying rice straw than corn straw,and the rice straw could be chosen in the main rice-growing areas.