Research on rapid propagation technology by tissue culture of Populus alba×P. alba var. pyramidalis

The rapid propagation technology was studied with sprouts of Populus alba×P.alba var.pyramidalis as explant.The results showed that the 6-BA 0.1 mg ·L-1 improved MS culture medium was the optimal medium for the primary culture stage,and the 6-BA0.1 mg·L-1+IAA0.5 mg·L-1 culture medium was the optimal medium for the subculture with 12~18 times of multiplication rate.The 1/2MS+NAA0.01 mg·L-1was the best culture medium for the rooting period,which could form root system after 15 d.