高尔基体
内体
液泡
小泡
甘露糖
甘露糖受体
免疫金标记
受体
6-磷酸甘露糖
细胞生物学
内质网
生物
生物物理学
亚细胞定位
化学
生物化学
细胞质
超微结构
巨噬细胞
膜
解剖
体外
生长因子
作者
J E Bleekemolen,Martin Stein,K von Figura,J W Slot,Hans J. Geuze
摘要
Using a semiquantitative immunogold technique on ultrathin cryosections, the in situ subcellular distributions of the cation-dependent, 46-kDa mannose 6-phosphate receptor (small MPR) and of the cation-independent, 270-kDa mannose 6-phosphate receptor (large MPR) were for the first time compared. U937 cells were chosen because of their relatively high content of both receptor species. Of each receptor, about 12% occurred at the cell surface, 2% in the Golgi stack, and about 25% in vacuoles resembling endosomal vacuoles. About half of both receptors was found in tubules, presumably belonging to endosomes and trans-Golgi reticulum. It was concluded that the distribution of the small and large MPR were roughly similar. The only exception was formed by electron-dense vesicles occurring in the trans-Golgi region and surrounding endosomes. Dense vesicles contained significantly less small MPR (7%) than large MPR (12%).
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