Research on the Tissue Culture and Plantlet Regeneration of Heracleum candicans

A system of tissue culture and plantlet regeneration of Heracleum candicans was established.The different combinations of plant growth regulators were added into MS medium to investigate their effects on tissue culture of leaf as explants and 100% callus was inducted on MS media with 2.0 mg/L 2,4-D and 0.5 mg/L 6-BA.And the most suitable subculture medium was MS with 2,4-D 2.0 mg/L and 6-BA 0.2 mg/L.Shoots were proliferated on MS media supplemented with 6-BA 2.0 mg/L and the rooting medium was 1/2 MS media added with NAA 0.2 mg/L and IAA 0.5 mg/L.