Taking Six soybean varieties as experimental material,the photosynthetic rate,stomatal canductance and transpiration rate of different leaf in different growth period were studied.The results showed that:The different varieties of soybean have different dynamic change of the photosynthetic rate in the whole growth period.OhioFG1 have high photosynthetic rate early days and later aging fate;HS93-4118 not high early days but have high photosynthetic rate later period;Tiefeng No.29 have high photosynthetic rate in the whole growth period.Therefore,when breeding varieties,we should not only according to instantaneous photosynthetic rate,but according to dynamic changes of photosynthetic rate in the whole growth period.After studying the photosynthetic rate,stomatal conductance and transpiration rate of different periods and sections in soybean,the results showed that the change of stomatal conductance and transpiration rate showed the same trend of photosynthetic rate.