Flavonoids from jujube pit were extracted with 60% percent ethanol and separated by AB-8 macroporous adsorption resin.DPPH free radical scavenging efficiency of flavonoids from jujube pit was studied first in this experiment. The results showed that the flavonoids from jujube pit have powerful scavenging efficiency to DPPH free radical. Its IC50 is 6.5μg/ml. The increase in the degree of DPPH free radical scavenging efficiency of flavonoids from jujube is concentration dependent.