According to its ability to synthesize 1,3-propanediol from glycerol,a species was isolated from the anoxic mud.The product of glycerol conversion was identified using GC-MS.The most active strain was able to convert up to 40 g/L of glycerol to(25.8 g/L) 1,3-propanediol in 96hr.The optimal conditions of fermentation were investigated.The yield of 1,3-propanediol was up to 47.3%.