Computer-Aided Rational Design Enhances 2- O -α-Glucosyl Glycerol Biosynthesis by Sucrose Phosphorylase in High-Viscosity Concentrated Substrates

-α-GG) production from 1.8 M sucrose and 2.16 M glycerol (a 208 g/L increase over the wild type) at 45 °C. This study highlights the industrial merits of rational integrated computational approaches, enabling efficient enzyme engineering for superior biocatalysis in high-viscosity systems.