Objective: Heat related illness is one of the leading causes of death in high school athletes. Proper hydration strategies have been shown to decrease heat related illness in college and professional athletes. However, limited research has been conducted on the hydration habits of high school athletes. The increase in high school sports participation has put more athletes at risk for dehydration. The purpose of the study is to investigate the hydration status and impact of an educational intervention on hydration habits of high school student athletes, by assessing their current understanding of hydration and monitoring their fluid intake, during practice. There is limited research on hydration habits of high school non-elite athletes. Methods: Sixteen female high school tennis players participated in this study. Data was collected on the players pre/post practice body weight and pre/post water bottle weight, during a pre-session, post session, and follow-up session. During the pre-session, researchers performed a series of educational sessions on hydration for the team and coaches. Researchers assessed hydration knowledge by giving participants replica hydration questionnaires at the beginning and conclusion of the study. A paired-sample t-test was used to compare differences in average weight change and percent weight change between the pre-session/post-session and presession/follow-up session. A paired-sample t-test was also used to analyze the average fluid ounces consumed and percent change in water bottle weight between pre-session/post-session and pre-session/follow-up session. A paired sample t-test was used to assess the differences between the pre/post hydration questionnaire, body weight, and fluid intake. Results: There were no significant differences in (p>0.05) between the pre-session and the postsession in regard to changes in percent body weight change or fluid intake. However, there were significant differences (p=0.06) in percent body weight change between the pre-session (0.00±0.50%) and the one-month follow-up session (-1.05 ±0.80%). Drinking habits during the follow-up session did not appear to be similar to the intervention period. Significant differences (p= 0.00) were noted between the pre-session (15.87± 6.00oz.) and follow-up session (0.00± 7.40oz.) in ounces of fluid consumed. The researchers found significant differences (p=0.00) between the percent weight change in water bottles between the pre-session (56.87±11.40oz.) and the follow-up session (27.25±15.17oz.). The were no significant differences between the results of the pre/post-hydration questionnaire. Conclusions: The findings from this study indicated that participants were more aware of their drinking habits during the invention period. Participants had similar drinking habits and saw less of a decrease in