粒体自噬
生物
李斯特菌溶血素O
单核细胞增生李斯特菌
细胞生物学
毒力因子
线粒体
自噬
细胞内寄生虫
李斯特菌
毒力
微生物学
细胞内
遗传学
细菌
基因
细胞凋亡
作者
Yifan Zhang,Yikun Yao,Xiaoliang Qiu,Guodong Wang,Zheng Hu,Siyuan Chen,Zhengxi Wu,Na Yuan,Hanchao Gao,Jianrong Wang,Houhui Song,Stephen E. Girardin,Youcun Qian
标识
DOI:10.1038/s41590-019-0324-2
摘要
Cells use mitophagy to remove damaged or unwanted mitochondria to maintain homeostasis. Here we report that the intracellular bacterial pathogen Listeria monocytogenes exploits host mitophagy to evade killing. We found that L. monocytogenes induced mitophagy in macrophages through the virulence factor listeriolysin O (LLO). We discovered that NLRX1, the only Nod-like receptor (NLR) family member with a mitochondrial targeting sequence, contains an LC3-interacting region (LIR) and directly associated with LC3 through the LIR. NLRX1 and its LIR motif were essential for L. monocytogenes-induced mitophagy. NLRX1 deficiency and use of a mitophagy inhibitor both increased mitochondrial production of reactive oxygen species and thereby suppressed the survival of L. monocytogenes. Mechanistically, L. monocytogenes and LLO induced oligomerization of NLRX1 to promote binding of its LIR motif to LC3 for induction of mitophagy. Our study identifies NLRX1 as a novel mitophagy receptor and discovers a previously unappreciated strategy used by pathogens to hijack a host cell homeostasis system for their survival.
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