肝细胞癌
转移
癌症研究
医学
肿瘤科
生物
内科学
癌症
作者
Lianqing Lou,Weiwei Ye,Yongxin Chen,Shuang Wu,Linzheng Jin,Jinke He,Xingfei Tao,Jinghong Zhu,Xiangyi Chen,Anmei Deng,Jinhe Wang
出处
期刊:Phytomedicine
[Elsevier]
日期:2012-05-01
卷期号:19 (7): 603-608
被引量:38
标识
DOI:10.1016/j.phymed.2012.01.003
摘要
Ardipusilloside I is a triterpene-saponin isolated from the Traditional Chinese Medicine Ardisia pusilla A. DC. Its effects and mechanism on invasion and metastasis of liver cancer cells are unclear. The human hepatocellular carcinoma cell line HepG2 and SMMC-7721 cells were treated with different doses of Ardipusilloside I. Cellular survival, in vitro migration and invasion were evaluated. In vivo metastatic abilities of the HCC cells were detected. We further investigated expression and phosphorylation of Mek, Erk and Akt by using Western blot. MMP2 and MMP9 activities were evaluated by gelatin zymography. E-cadherin expression, Rac1 and Cdc42 activities were examined by Western blot and pull-down assay. Ardipusilloside I inhibited invasion and metastasis of HCC cells both in vitro and in vivo by reducing the protein expressions of metalloproteinase (MMP)-9 and MMP2 proteins. Ardipusilloside I activated Rac1 that enhanced E-cadherin activity and resulted in significantly less metastasis. Our findings indicate that Ardipusilloside I has the potential of inhibition of liver cancer survival, invasion and metastasis both in vitro and in vivo.
科研通智能强力驱动
Strongly Powered by AbleSci AI