趋磁细菌
脂质体
药物输送
生物相容性
生物物理学
细胞毒性
靶向给药
毒品携带者
纳米技术
化学
药品
碳二亚胺
细菌
药理学
体外
材料科学
生物
生物化学
有机化学
遗传学
作者
Samira Taherkhani,Mohammad Mohammadi,Jamal Daoud,Sylvain Martel,Maryam Tabrizian
出处
期刊:ACS Nano
[American Chemical Society]
日期:2014-04-08
卷期号:8 (5): 5049-5060
被引量:200
摘要
The targeted and effective delivery of therapeutic agents remains an unmet goal in the field of controlled release systems. Magnetococcus marinus MC-1 magnetotactic bacteria (MTB) are investigated as potential therapeutic carriers. By combining directional magnetotaxis-microaerophilic control of these self-propelled agents, a larger amount of therapeutics can be delivered surpassing the diffusion limits of large drug molecules toward hard-to-treat hypoxic regions in solid tumors. The potential benefits of these carriers emphasize the need to develop an adequate method to attach therapeutic cargos, such as drug-loaded nanoliposomes, without substantially affecting the cell's ability to act as delivery agents. In this study, we report on a strategy for the attachment of liposomes to MTB (MTB-LP) through carbodiimide chemistry. The attachment efficacy, motility, and magnetic response of the MTB-LP were investigated. Results confirm that a substantial number of nanoliposomes (∼70) are efficiently linked with MTB without compromising functionality and motility. Cytotoxicity assays using three different cell types (J774, NIH/3T3, and Colo205) reveal that liposomal attachments to MTB formulation improve the biocompatibility of MTB, whereas attachment does not interfere with liposomal uptake.
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