纤维连接蛋白
成纤维细胞
材料科学
真皮成纤维细胞
生物医学工程
粘附
钛
钛合金
细胞粘附
体外
细胞
复合材料
合金
医学
冶金
化学
生物化学
作者
CJ Pendegrass,C. A. Middleton,David Gordon,Josh Jacob,Gordon Blunn
摘要
Abstract The success of intraosseous transcutaneous amputation prostheses (ITAP) relies on soft tissue attachment to prevent infection which leads to implant failure. Fibronectin (Fn) has been shown to enhance dermal fibroblast attachment in vitro , however measurement of cell attachment strength has been indirect; using cell area and immunolocalization of focal adhesion components. In this study, we have developed a flow apparatus to assess the biophysical strength of cell attachment to biomaterials used in ITAP. We have demonstrated that dermal fibroblast attachment strength increases significantly up to 96 h and that data from direct and indirect methods of assessing cell attachment strength have a significant positive correlation. Additionally, we have used direct and indirect assessment methods to demonstrate that dermal fibroblast attachment strength is significantly greater on fibronectin‐coated titanium alloy compared with uncoated controls at 1, 4, and 24 hours. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
科研通智能强力驱动
Strongly Powered by AbleSci AI