脂毒性
脂肪变性
脂肪肝
脂质过氧化
氧化应激
甘油三酯
内分泌学
内科学
化学
β氧化
脂滴
非酒精性脂肪肝
脂肪酸
脂质代谢
生物化学
医学
胆固醇
胰岛素抵抗
糖尿病
疾病
作者
Jie-wei Wang,Xingyong Wan,Hongyan Zhu,Chao Lü,Wei-lai Yu,Zhe Shen,Youming Li
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2014-04-30
卷期号:9 (4): e96124-e96124
被引量:12
标识
DOI:10.1371/journal.pone.0096124
摘要
Nonalcoholic fatty liver disease (NAFLD) is increasingly regarded as a hepatic manifestation of metabolic syndrome. Though with high prevalence, the mechanism is poorly understood. This study aimed to investigate the effects of p21 on free fatty acid (FFA)-induced steatosis in L02 cells. We therefore analyzed the L02 cells with MG132 and siRNA treatment for different expression of p21 related to lipid accumulation and lipotoxicity. Cellular total lipid was stained by Oil Red O, while triglyceride content, cytotoxicity assays, lipid peroxidation markers and anti-oxidation levels were measured by enzymatic kits. Treatment with 1 mM FFA for 48 hr induced magnificent intracellular lipid accumulation and increased oxidative stress in p21 overload L02 cells compared to that in p21 knockdown L02 cells. By increasing oxidative stress and peroxidation, p21 accelerates FFA-induced lipotoxic effect in L02 cells and might provide information about potentially new targets for drug development and treatments of NAFLD.
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