流式细胞术
程序性细胞死亡
细胞
神经退行性变
细胞生物学
化学
细胞仪
癌细胞
癌症研究
细胞凋亡
生物
免疫学
癌症
生物化学
医学
病理
遗传学
疾病
作者
Daishi Chen,Ilker Y. Eyüpoglu,Nicolai Ε. Savaskan
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 71-77
被引量:18
标识
DOI:10.1007/978-1-4939-6960-9_6
摘要
Cell death and its recently discovered regulated form ferroptosis are characterized by distinct morphological, electrophysiological, and pharmacological features. In particular ferroptosis can be induced by experimental compounds and clinical drugs (i.e., erastin, sulfasalazine, sorafenib, and artesunate) in various cell types and cancer cells. Pharmacologically, this cell death process can be inhibited by iron chelators and lipid peroxidation inhibitors. Relevance of this specific cell death form has been found in different pathological conditions such as cancer, neurotoxicity, neurodegeneration, and ischemia. Distinguishing cell viability and cell death is essential for experimental and clinical applications and a key component in flow cytometry experiments. Dead cells can compromise the integrity of the data by nonspecific binding of antibodies and dyes. Therefore it is essential that dead cells are robustly and reproducibly identified and characterized by means of cytometry application. Here we describe a procedure to detect and quantify cell death and its specific form ferroptosis based on standard flow cytometry techniques.
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