Development of New Laccase-Mediators for Pulp Bleaching

Analogs of N-hydroxyacetanilide (NHA), one of the best laccase-mediators reported so far, were prepared and evaluated as laccase-mediators for pulp bleaching. Kinetic properties of laccase for these NHA analogs were also determined. It was found that the kinetic properties of laccase such as Km and kcat are significantly influenced by the composition of laccase-mediators. It was demonstrated for the first time that more effective laccase-mediators could be developed through chemical modification of another laccase-mediator.