Development of New Laccase-Mediators for Pulp Bleaching

Analogs of N-hydroxyacetanilide (NHA), one of the best laccase-mediators reported so far, were prepared and evaluated as laccase-mediators for pulp bleaching. Kinetic properties of laccase for these NHA analogs were also determined. It was found that the kinetic properties of laccase such as K m and k cat are significantly influenced by the composition of laccase-mediators. It was demonstrated for the first time that more effective laccase-mediators could be developed through chemical modification of another laccase-mediator.