青蒿
青蒿素
脱落酸
转录因子
基因
生物
RNA干扰
生物合成
转录组
蒿属
化学
倍半萜内酯
细胞生物学
生物化学
基因表达
恶性疟原虫
免疫学
疟疾
核糖核酸
作者
Guoping Shu,Kexuan Tang,Mingyuan Yuan,Ning Wei,Fangyuan Zhang,Chunxian Yang,Xiaozhong Lan,Min Chen,Kexuan Tang,Lan Xiang,Zhihua Liao
标识
DOI:10.1016/j.apsb.2021.09.026
摘要
Artemisia annua is the main natural source of artemisinin production. In A. annua, extended drought stress severely reduces its biomass and artemisinin production while short-term water-withholding or abscisic acid (ABA) treatment can increase artemisinin biosynthesis. ABA-responsive transcription factor AabZIP1 and JA signaling AaMYC2 have been shown in separate studies to promote artemisinin production by targeting several artemisinin biosynthesis genes. Here, we found AabZIP1 promote the expression of multiple artemisinin biosynthesis genes including AaDBR2 and AaALDH1, which AabZIP1 does not directly activate. Subsequently, it was found that AabZIP1 up-regulates AaMYC2 expression through direct binding to its promoter, and that AaMYC2 binds to the promoter of AaALDH1 to activate its transcription. In addition, AabZIP1 directly transactivates wax biosynthesis genes AaCER1 and AaCYP86A1. The biosynthesis of artemisinin and cuticular wax and the tolerance of drought stress were significantly increased by AabZIP1 overexpression, whereas they were significantly decreased in RNAi-AabZIP1 plants. Collectively, we have uncovered the AabZIP1-AaMYC2 transcriptional module as a point of cross-talk between ABA and JA signaling in artemisinin biosynthesis, which may have general implications. We have also identified AabZIP1 as a promising candidate gene for the development of A. annua plants with high artemisinin content and drought tolerance in metabolic engineering breeding.
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