费斯特共振能量转移
化学
DNA
生物传感器
荧光
堆积
量子点
检出限
杂交探针
接受者
生物物理学
结晶学
纳米技术
生物化学
色谱法
有机化学
材料科学
生物
物理
量子力学
凝聚态物理
作者
Lushan Liang,Meng Chang Chen,Yanli Tong,Wei-Guo Tan,Zuanguang Chen
标识
DOI:10.1016/j.aca.2021.339090
摘要
Herein, a universal fluorescent biosensor was developed for detecting Mycobacterium Tuberculosis (MTB) specific insertion sequence IS6110 gene fragment based on Förster resonance energy transfer (FRET) strategy. CdTe quantum dots (QDs), with excellent luminous performance, were used to label single-stranded DNA (ssDNA) as fluorescence donor (QDs-DNA), in which the ssDNA was complementary to the IS6110 gene fragment. A new type of two-dimensional metal-organic framework (Cu-TCPP) was served as an acceptor. The Cu-TCPP exhibited a higher affinity towards ssDNA than double-stranded DNA (dsDNA). In the absence of targets, the fluorescence of QDs-DNA was quenched - due to the π-π stacking interactions between Cu-TCPP and ssDNA. Otherwise, QDs-DNA hybridized with the target to form a double helix and the fluorescence maintained in a target-concentration dependent manner. Excess QDs-DNA would be quenched and produced negligible background signal. The fluorescent sensor possessed a linear range from 0.05 nM to 1.0 nM with a low detection limit of 35 pM. Furthermore, we successfully applied this biosensing system to detect clinical sputum samples. This method displayed high sensitivity, specificity and great potentials in the early diagnosis of Tuberculosis.
科研通智能强力驱动
Strongly Powered by AbleSci AI