车前草
银杏
雄激素受体
生物测定
受体
传统医学
药理学
体外
激素
雌激素受体
内分泌系统
生物
报告基因
化学
植物
医学
内科学
内分泌学
生物化学
基因表达
癌症
乳腺癌
基因
前列腺癌
遗传学
作者
Macarena Real,José-Manuel Molina-Molina,J. de Jesus Jimenez,Horacio R Diéguez,Mariana F. Fernández,Nicolás Olea
标识
DOI:10.1080/19440049.2015.1071922
摘要
Medicinal plants are widely used for the treatment of diseases and for the development of new drugs. This study was designed to determine the presence of hormone-like activities dependent on the activation of human estrogen receptor alpha (hERa) and/or androgen receptor (hAR) in methanol extracts prepared from three medicinal plants historically and currently used for therapeutic purposes: Ginkgo biloba leaves (GBL), Elettaria cardamomum seeds (ECS) and Plantago ovata seeds (POS). After a solid–liquid extraction (SLE) step, their effects on hERa function were assessed in MCF-7 breast cancer cells using the E-Screen bioassay, and their ability to induce hAR-mediated reporter gene expression was evaluated using the androgen-sensitive stable prostatic PALM cell line. Unlike POS extracts, GBL and ECS extracts showed estrogenic (0.07 and 0.20 nM E2Eq mg–1, respectively) and anti-estrogenic (0.01 and 0.02 μM ICI182780Eq mg–1, respectively) activities. ECS extracts evidenced androgenic activity (0.30 nM R1881Eq mg–1) and POS extracts anti-androgenic activity (22.30 μM ProcEq mg–1). According to these findings, these plant extracts may interfere with the endocrine system via one or more hormonal receptors, and further investigation is warranted into their role as endocrine disrupters in humans.
科研通智能强力驱动
Strongly Powered by AbleSci AI