A Novel Method for the Analysis of Soluble Carbohydrates from Cistanche deserticola

Objective: A comprehensive monosaccharide quantifying method was developed in this article. Monosaccharides (including glucose, arabinose, galacturonic acid, galactose, mannose, rhamnose and xylose) were analyzed by RP-HPLC-DAD method. Meanwhile, mannitol and fructose were calculated based on peak areas obtained by HPIEC-RID and corresponding data obtained by RP-HPLC-DAD. Monosaccharide compositions of CDSC were analyzed, nine kinds of monosacchardes (glucose, arabinose, galctose, galacturonic acid, mannose, rhamnose, xylose, fructose and mannitol) in were quantified. Keywords: Cistanche deserticola Y. C. Ma, soluble carbohydrates, monosaccharide compositions, high performance liquid chromatography, high-performance ion exclusion chromatography, herbal medicine.