DSCImageCalc is free software for calculating similarity coefficients on segmented images. It is written in Visual BASIC .NET and is available at: https://doi.org/10.6084/m9.figshare.894428. It presently calculates Sørensen-Dice Coefficient, Jaccard Coefficient, Proportional Agreement, Cohen’s Kappa, Goodman & Kruskal’s Gamma, and Rogot-Goldberg Agreement. It also calculates segment centroids and the distance between them. Whilst it was originally designed for calculating Dice coefficients on segmentations of ultrasound images, it has potential for re-use in any field where similarity of image segments is to be analysed; predominantly medical radiology and ecology.