聚腺苷酸
核糖核酸
计算生物学
生物
信使核糖核酸
基因
基因表达
DNA测序
分子生物学
遗传学
作者
Pamela Moll,Michael Ante,Alexander Seitz,Torsten Reda
出处
期刊:Nature Methods
[Nature Portfolio]
日期:2014-11-25
卷期号:11 (12): i-iii
被引量:328
摘要
QuantSeq provides an easy protocol to generate highly strand-specific next-generation sequencing (NGS) libraries close to the 3′ end of polyadenylated RNAs within 4.5 h. Only one fragment per transcript is generated, directly linking the number of reads mapping to a gene to its expression. QuantSeq reduces data analysis time and enables a higher level of multiplexing per run. QuantSeq is the RNA sample preparation method for accurate and affordable gene expression measurement.
科研通智能强力驱动
Strongly Powered by AbleSci AI