化学
动力学
聚合物
氨
活化能
氨基酸
牛血清白蛋白
色谱法
有机化学
生物化学
量子力学
物理
作者
Taku Michael Aida,Minori Oshima,Richard L. Smith
出处
期刊:ACS Sustainable Chemistry & Engineering
[American Chemical Society]
日期:2017-08-18
卷期号:5 (9): 7709-7715
被引量:19
标识
DOI:10.1021/acssuschemeng.7b01146
摘要
Reaction of bovine serum albumin (BSA) protein in high-temperature (200−260 °C) water (HTW) with fast heating rates (ca. 135−180 K·s–1) without acid or base additives gives high-molecular-weight (1500–8300 Da) peptides with minimal formation of amino acids and ammonia. The decrease in the number-average molecular weight of peptides after HTW treatment of BSA could be described by a kinetic model based on random scission mechanism of the polymer chain. Reaction of BSA in HTW under identical conditions with slow heating rates (ca. 0.25 K·s–1) gives peptides of low molecular weight with formation of amino acids and ammonia for which the kinetics could not be described by a random scission mechanism. The activation energy determined for the conversion of BSA into high-molecular-weight peptides with fast heating rates in high-temperature water was 16.4 kJ·mol–1. Reaction of proteins in high-temperature water with fast heating rates inhibits initial aggregation that occurs during slow heating rates and allows controlled conversion of the denatured polymer chain into high-molecular-weight peptides.
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