The present study is focused on the determination of the 2-phenylethanol production by yeasts genus Kluyveromyces. The most productive strain was K. marxianus UCM Y-305. It can be concluded that 2-phenylethanol production by K. marxianus UCM Y-305 can be improved from 0.0268 g/l to 0.37 g/l by selecting the suitable cultivating conditions.