Expression and Purification of Secreted form TNF-related Activation-induced Cytokine in E.coli as a Maltose Binding Protein Fusion

Purpose To express and purify the protein of sTRANCE(secreted form TNF related activation induced cytokine). Methods sTRANCE gene was cloned into expressed vector PMAL c2x and the fusion protein MBP sTRANCE was expressed in E.coli . Results The molecular weights of MBP sTRANCE was 70 000.Western blot analysis showed the fusion protein presented the specific MBP antigenicity.MBP sTANCE fusion protein were purified by amylose resin affinity chromatography and showed to be homogeneity in SDS PAGE. Conclusions The MBP sTRANCE fusion protein was obtained and maybe suitable for studying biological functions and potential utility in neoplasm treatment.