Objective To study the antimicrobial susceptibility and distribution of minimal inhibitory concentration of the clinical isolated Stenotrophomonas maltophilia . Methods The Stenotrophomonas maltophilia was identified and detected with Bio merieux VITEK Ⅱ automated microbial indentification system. Results The antimicrobial susceptibility rates to Stenotrophomonas maltophilia of sulfamethoxazole trimethoprim (SMZ/TMP) and levoflixacin were 79.31% and 72.4% respectively, and it was lower comparing to other antibiotics. The minimal inhibitory concentraction of 79% Stenotrophomonas maltophilia to SMZ/TMP was ≤4 μg/ml. The minimal inhibitory concentraction of 93% Stenotrophomonas maltophilia to levoflixacin was ≤4 μg/ml. Conclusions Stenotrophomonas maltophilia mainly caused respiratory tract infection in patients and mulitidrug resistance is serious. In treating Stenotrophomonas maltophilia infection, antimicrobial drugs should be selected according to the results of antimicrobial susceptibility test and minimal inhibitory concentration.