Modification of soybean protein concentrate by enzymatic hydrolysis

The enzymatic hydrolysis has the advantages of highpurity product and mild reaction,so the soybean protein concentrate was modified by enzymatic hydrolysis in this research.Using the nitrogen solution index as a checking standard,different enzymes in hydrolyzing of soybean proteins concentrate,including papain,neutral proteinase 1398 and the mixture of both exzymes were compared.The results showed that the hydrolysis effect of the mixture enzyme was better than that of single enzyme through single-element and orthogonal experiment.The optimum conditions is substrate consistency 6.5 %,pH 6.5,temperature 55℃,time 3 hours,mixture enzyme activity 5 000 U/g.The nitrogen solution index can be up to 81.94 % under the optimum conditions.It may have a rather bright future in industralization.