Phosphorylation Modification of Polysaccharides from Entermorpha

This study was undertaken to optimize the phosphorylation of Enteromorph polysaccharides prepared by hot water extraction,deproteinization,decolorization,and sequential chromatographic purification on DEA-Sepharose Fast Flow and Sephadex G-100 columns.Phosphate group content in phosphorylated products was investigated with respect to reaction conditions including temperature,reaction time,pH and phosphorylation agent dosage(sodium tripolyphosphate-sodium trimetaphosphate at a mass ratio of 6:1) by one-factor-at-a-time experiments and subsequent response surface analysis.The optimal phosphorylation conditions were pH 9.0,temperature 80 ℃ and phosphorylation agent dosage 0.10 g/mL for a reaction duration of 5 h.Under these conditions,the phosphate group content in phosphorylated products was 14.40%.