Determination of Gastrodin in Gastrodia eleta Bl. By RP-HPLC

Objective To constitute the analytical method for determination gastrodin in Gastrodia elata Bl. by RP-HPLC. Method Optimize the extracting and separate method to get the superior spectrum for quantilizing gastrodin by standard curve. HPLC analysis was carried out with C18 column and methanol-0. l%acetic acid (5 : 95) as the mobile phase. The detection wavelength was 270 nm. Result Twelve peaks can be used for quantity, in which gastrodin was determinated with 0. 60-2. 41 μg (r=0. 9999) as the linear range and 97. 85% (RSD=1. 17%, n = 5) as the average recovery. Conclusion The method is simple, reliable and good recurrence. It can be used to determine gastrodin in Gastrodia elata Bl. , and other water soluble constituents as well.