小泡
化学
生物物理学
电穿孔
脂质双层
荧光
膜
二价
超分子化学
分子
生物化学
有机化学
生物
物理
量子力学
基因
作者
Chloé Mauroy,Isabelle Rico–Lattes,Justin Teissié,Marie‐Pierre Rols
出处
期刊:Langmuir
[American Chemical Society]
日期:2015-10-30
卷期号:31 (44): 12215-12222
被引量:18
标识
DOI:10.1021/acs.langmuir.5b03090
摘要
Biological membranes are weakly permeable to hydrophilic molecules and ions and electric pulses can induce their transient permeabilization, but this process is not well characterized. We directly assay the electropermeabilization process, on the minimum model of lipid vesicles, by using a highly sensitive fluorescence method based on manganese ion transport. The approach gives access, at the single-lipid self-assembly level, to the transmembrane potential needed to detect divalent ion permeabilization on supramolecular giant unilamellar lipid vesicles. The critical values are strongly dependent on the lipid composition and are observed to vary from 10 to 150 mV. These values appear to be much lower than those previously reported in the literature for cells and vesicles. The detection method appears to be a decisive parameter as it is controlled by the transport of the reporter dye. We also provide evidence that the electropermeabilization process is a transient transition of the lipid self-organization due to the loss of assembly cohesion induced by bioelectrochemical perturbations of the zwitterionic interface with the solution.
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