Chalcone synthase(CHS) and chalcone isomerase(CHI) are the key enzymes controlling the biosynthesis of flavonoid,for CHS can catalyze its substrate into naringenin-chalcone,which will sequentially be isomerized into flavonoid compounds by CHI.These two enzymes exist widely in most plants,and involve the biosynthesis of preventive products from phenylpropanoid pathway,hence playing an important role in the anti-fungus mechanism,stress resistance,cell development and differentiation,pigment accumulation,and exogenous gene expression in plants. In this paper,the structural traits of CHS and CHI,gene cloning and sequence characteristics,gene expression and regulation,and its transgenic application, are systematically summarized,and feasible suggestions for further researches are presented.