Study on the Cell-immobilization Technique in Saccharomyces cerevisiae

Saccharomyces cerevisiae cell was immobilized by entrapment with alginic -agar. Liquid medium was fermentated by immobilized Saccharomyces cerevisiae, the singal factor of immobilized Saccharomyces cerevisiae was analysed with alcohol degree as the index. Then the optimum condition of immobilized yeast was confirmed by the orthogonal experiment. The results showed that the optimum conditions of immobilized Saccharomyces cerevisiae were determined as follows: the concentration of carrier1.4g·40mL-1、proportion of composite carrier 1∶1, temperature 46℃.