Purification and Identification of Oligodendrocyte-type-2-astrocyte Progenitor Cells in Vitro

Aim:To explore the method of culture and purification of oligodendrocyte-type-2-astrocyte progenitor cells derived from cortex of new-born rats and identification.Methods:Mixed glial cells derived from cortex of neonate rats were cultured and oligodendrocyte-type-2-astrocyte progenitor cells were purified by modified method of shaking isolation.The purified cells were then induced to differentiation and identified by immunochemical staining.Results:oligodendrocyte-type-2-astrocyte progenitor cells exhibit double positive staining of A2B5 and Nestin,which have the potential ability to differentiate into astrocytes and oligodendrocytes.The purification of isolated cells can reach over 90%.Conclusion:High purification oligodendrocyte-type-2-astrocyte progenitor cells can be acquired by low rate shaking combined with secondary plated method,so it is a simple and high effective method for the purification of oligodendrocyte-type-2-astrocyte progenitor cells.