硒
嗜麦芽窄食单胞菌
化学
生物转化
生物发生
寡养单胞菌
纳米颗粒
生物化学
细菌
生物
酶
纳米技术
有机化学
材料科学
铜绿假单胞菌
基因
遗传学
16S核糖体RNA
作者
Silvia Lampis,Emanuele Zonaro,Cristina Bertolini,Daniela Cecconi,Francesca Monti,Massimo Micaroni,Raymond J. Turner,Catherine Butler,Giovanni Vallini
标识
DOI:10.1016/j.jhazmat.2016.02.035
摘要
A putative biosynthetic mechanism for selenium nanoparticles (SeNPs) and efficient reduction of selenite (SeO32−) in the bacterial strain Stenotrophomonas maltophilia SeITE02 are addressed here on the basis of information gained by a combined approach relying on a set of physiological, chemical/biochemical, microscopy, and proteomic analyses. S. maltophilia SeITE02 is demonstrated to efficiently transform selenite into elemental selenium (Se°) by reducing 100% of 0.5 mM of this toxic oxyanion to Se° nanoparticles within 48 h growth, in liquid medium. Since the selenite reducing activity was detected in the cytoplasmic protein fraction, while biogenic SeNPs showed mainly extracellular localization, a releasing mechanism of SeNPs from the intracellular environment is hypothesized. SeNPs appeared spherical in shape and with size ranging from 160 nm to 250 nm, depending on the age of the cultures. Proteomic analysis carried out on the cytoplasmic fraction identified an alcohol dehydrogenase homolog, conceivably correlated with the biogenesis of SeNPs. Finally, by Fourier Transformed Infrared Spectrometry, protein and lipid residues were detected on the surface of biogenic SeNPs. Eventually, this strain might be efficaciously exploited for the remediation of selenite-contaminated environmental matrices due to its high SeO32− reducing efficiency. Biogenic SeNPs may also be considered for technological applications in different fields.
科研通智能强力驱动
Strongly Powered by AbleSci AI