显微镜
材料科学
生物物理学
荧光显微镜
化学
显微镜
扫描电镜
超分辨显微术
作者
Yasin C. Durmaz,Alexandra Goetz,F. Keilmann
出处
期刊:International Conference on Infrared, Millimeter, and Terahertz Waves
日期:2019-09-01
被引量:3
标识
DOI:10.1109/irmmw-thz.2019.8873819
摘要
We report progress towards sealing alive cells in their native, wet environment by single-layer graphene, where graphene stabilizes the cell by tightly conforming with its surface and thus enables topographic AFM maps of the cell's surface. Furthermore, graphene's transparency to infrared near-fields enables local chemical recognition, at nominally 20-nm spatial resolution, by scattering scanning mid-infrared microscopy (s-SNOM) and spectroscopy (nano-FTIR). Here we demonstrate first infrared nanoscopy results of the surface of native E. Coli cells that show spatial and spectral contrasts. Our study opens a new door into characterizing subcellular structures and components of cells and of fresh tissue sections. Infrared nanoscopy is certainly free of any need for labelling as in super-resolution fluorescence microscopy, and free of fixation and metallization procedures as in electron microscopy that kill and distort cells.
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