生物
草甘膦
转基因水稻
转基因
甘氨酸
转基因作物
杀菌剂
激发子
生物技术
植物
基因
生物化学
氨基酸
作者
Sahil Mehta,Aundy Kumar,V. Mohan Murali Achary,Ganesan Prakash,Neelmani Rathi,Asmita Singh,Kuleshwar Prasad Sahu,Shambhu Krishan Lal,Twarita Das,Malireddy K. Reddy
出处
期刊:Plant Science
[Elsevier]
日期:2021-10-01
卷期号:311: 111009-111009
被引量:7
标识
DOI:10.1016/j.plantsci.2021.111009
摘要
Weeds, pests, and pathogens are among the pre-harvest constraints in rice farming across rice-growing countries. For weed management, manual weeding and herbicides are widely practiced. Among the herbicides, glyphosate [N-(phosphonomethyl) glycine] is a broad-spectrum systemic chemical extensively used in agriculture. Being a competitive structural analog to phosphoenolpyruvate, it selectively inhibits the conserved 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme required for the biosynthesis of aromatic amino acids and essential metabolites in eukaryotes and prokaryotes. In the present study, we investigated the antifungal and defense elicitor activity of glyphosate against Magnaporthe oryzae on transgenic-rice overexpressing a glyphosate-resistance OsEPSPS gene (T173I + P177S; TIPS OsmEPSPS) for blast disease management. The glyphosate foliar spray on OsmEPSPS transgenic rice lines showed both prophylactic and curative suppression of blast disease comparable to a blasticide, tricyclazole. The glyphosate displayed direct antifungal activity on Magnaporthe oryzae as well as enhanced the levels of antioxidant enzymes and photosynthetic pigments in rice. However, the genes associated with phytohormones-mediated defense (OsPAD4, OsNPR1.3, and OsFMO) and innate immunity pathway (OsCEBiP and OsCERK1) were found repressed upon glyphosate spray. Altogether, the current study is the first report highlighting the overexpression of a crop-specific TIPS mutation in conjugation with glyphosate application showing potential for blast disease management in rice cultivation.
科研通智能强力驱动
Strongly Powered by AbleSci AI