生物
聚糖
病毒
计算生物学
腺相关病毒
病毒学
免疫系统
基因
病毒复制
病毒进入
转导(生物物理学)
载体(分子生物学)
遗传学
糖蛋白
生物物理学
重组DNA
作者
Nancy Meyer,Michael S. Chapman
标识
DOI:10.1016/j.tim.2021.09.005
摘要
Adeno-associated virus (AAV) is the leading vector in emerging treatments of inherited diseases. Higher transduction efficiencies and cellular specificity are required for broader clinical application, motivating investigations of virus-host molecular interactions during cell entry. High-throughput methods are identifying host proteins more comprehensively, with subsequent molecular studies revealing unanticipated complexity and serotype specificity. Cryogenic electron microscopy (cryo-EM) provides a path towards structural details of these sometimes heterogeneous virus-host complexes, and is poised to illuminate more fully the steps in entry. Here presented, is progress in understanding the distinct steps of glycan attachment, and receptor-mediated entry/trafficking. Comparison with structures of antibody complexes provides new insights on immune neutralization with implications for the design of improved gene therapy vectors.
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