溶血素
溶血
微生物学
毒力
金黄色葡萄球菌
琼脂
琼脂平板
生物
细菌
免疫学
生物化学
基因
遗传学
作者
Miranda J. Ridder,Seth M. Daly,Pamela R. Hall,Jeffrey L. Bose
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 25-30
被引量:5
标识
DOI:10.1007/978-1-0716-1550-8_4
摘要
Many strains of Staphylococcus aureus produce a variety of cytolysins that target many different cell types to both fight the immune system and acquire nutrients. This includes hemolysins which destroy erythrocytes and are well studied virulence factors. Traditionally, hemolysin activity is measured on blood agar plates due to the simplicity of the assay. While this is telling, it cannot encapsulate the full story because S. aureus is known to behave differently in broth and on agar. Furthermore, plate-based assays are primarily semiquantitative and often a more accurate determination of hemolytic potential is needed to discern differences between strains. Here, we describe a method to quantify hemolysin activity from broth or similarly grown cells.
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