First report of Metschnikowia bicuspidata infection in Chinese grass shrimp ( Palaemonetes sinensis ) in China

Transboundary and Emerging DiseasesVolume 69, Issue 5 p. 3133-3141 SHORT COMMUNICATION First report of Metschnikowia bicuspidata infection in Chinese grass shrimp (Palaemonetes sinensis) in China Gangnan Cao, Gangnan Cao Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorJie Bao, Jie Bao Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorChengcheng Feng, Chengcheng Feng Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorXiaodong Li, Xiaodong Li Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorYuxi Lang, Yuxi Lang Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorYuenan Xing, Yuenan Xing Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorHongbo Jiang, Corresponding Author Hongbo Jiang jianghb@syau.edu.cn orcid.org/0000-0001-5166-9298 Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. China Correspondence Hongbo Jiang, Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang 110866, P. R. China. Email: jianghb@syau.edu.cnSearch for more papers by this author Gangnan Cao, Gangnan Cao Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorJie Bao, Jie Bao Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorChengcheng Feng, Chengcheng Feng Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorXiaodong Li, Xiaodong Li Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorYuxi Lang, Yuxi Lang Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorYuenan Xing, Yuenan Xing Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. ChinaSearch for more papers by this authorHongbo Jiang, Corresponding Author Hongbo Jiang jianghb@syau.edu.cn orcid.org/0000-0001-5166-9298 Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, P. R. China Correspondence Hongbo Jiang, Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang 110866, P. R. China. Email: jianghb@syau.edu.cnSearch for more papers by this author First published: 25 January 2022 https://doi.org/10.1111/tbed.14460Citations: 3 Authors Gangnan Cao and Jie Bao have contributed equally to this work. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The Chinese grass shrimp (Palaemonetes sinensis) was found with a white turbidity appearance in the Panjin area. After dissection, typical symptoms of milky disease with hemolymph emulsification and noncoagulation were observed; however, the pathogen was unknown. In this study, we aimed to isolate the pathogen of the diseased P. sinensis. We found that the pathogen could grow on the fungal medium Bengal red, and microscopic examination showed that it reproduced by budding. Molecular identification of the isolated and purified yeast strain LNMB2021 based on 26S rDNA sequence showed that the pathogenic pathogen was Metschnikowia bicuspidata (GenBank OK094821), with 98.74% homology with M. bicuspidata strain LNES0119 (GenBank OK073903) and 98.56% with M. bicuspidata strain Liao (GenBank MT856369). The results of an artificial infection test showed that M. bicuspidata caused the same clinical symptoms in P. sinensis, and the isolated pathogen was still the same, which proved that P. sinensis was a new host of M. bicuspidata. Histopathological analysis showed that there were obvious pathological changes in the hepatopancreas and muscle tissue of the diseased P. sinensis. Identification of the pathogen is essential for the prevention and control of the disease and the healthy culture of P. sinensis. Furthermore, considering the transmissibility and cross-host transmission of M. bicuspidata, its risk of infecting other aquatic animals deserves high attention. CONFLICT OF INTEREST The authors declare no conflict of interest. Open Research DATA AVAILABILITY STATEMENT All data included in this study are available upon request from the corresponding author. Citing Literature Volume69, Issue5September 2022Pages 3133-3141 RelatedInformation